Mitochondria : Discovery , Morphology & Chemical Composition
1. Discovery & Occurance
- First observed by Kölliker in 1880 in the striated muscles of insects.
- Mitochondria were actually discovered by W. Fleming and R. Altman.
- According to Fleming (in 1882), mitochondria are thread-like structures, which he named Phyla.
- Altman observed them in cells in 1890 and referred to them as granular particles and named them Bioplasts. That is, a thread made of granules.
- Mitochondria was named by C. Benda. Mitochondria is made up of two words, Mitos and Chondrion. Mitos means thread, and Chondria means granule. That is, a thread made of granules.
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| Mitochondria |
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- Ben-sley & Hoerr isolated mitochondria from liver cells in 1934.
- Their presence in plant cells was discovered in 1904 by F. Meves in a plant called Nymphaea.
- Michalis in 1898 discovered that mitochondria plays an important role in respiration and told that these are the centers of the Cellular Respiration.
- All the mitochondria present in a single cell are collectively called a Chondriosome.
- These are also known as Chondrioplast , Plasmosome , Plastosome and Plastochondrian.
- Except bacteria and blue-green algae , Mitochondria are present in every cells of plants and animals of living organisms.
2. Morphology Of The Mitochondria
- Mitochondria seems like Rod-shaped , Filamentous , Spherical and Granular .
- The morphology of mitochondria is different in every cell.
- The perimeter of the mitochondria is 0.5 - 2 µm. However, depending on their ability to function in certain conditions, they are found up to 7 µm in size.
- The perimeter of the mitochondria is 0.5 - 2 µm.
- The average length of Mitochondria is 3 - 5 µm and the diameter is 0.5 - 2 µm .
- Depending on the cell's function and volume, the number of mitochondria in a cell can range from 50 to 50,000. Animal cells contain more mitochondria than plant cells.
- Algae cells contains only one mitochondria.
- Yeast cells contains less than 10 mitochondria.
- Amoeba contains approximately 50,000 mitochondria.
- Liver cells possess 1000 - 1600 mitochondria. Liver cells of Rat Possess 1000 to 2500 mitochondria.
- Sea urchin eggs have 1,50,000 to 3,000,000 mitochondria.
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3. Chemical Composition
- Mitochondria contain 65-70% proteins, of which 30% are structural proteins and 40% are functional proteins.
- Mitochondria contain 25-30% lipids, of which 90% are phospholipids and the remaining 10% are Carotenoids, Cholesterol, Vitamin E, etc.
- Sulphur, Iron, Copper, Calcium, Magnesium and some other Vitamins are also found in trace amounts.
- 70 S Ribosomes are found in mitochondria.
- DNA , RNA and RNA Polymerase etc. are also present in mitochondria.
|
Sr. No. |
Chemical Substances |
Quantity ( in % ) |
|
|
01 |
Protein |
65 – 70 % |
|
|
i. |
Structural Protein |
30 % |
|
|
ii. |
Enzymatic Protein |
40 % |
|
|
02 |
Lipid |
25 – 30 % |
|
|
i. |
Phospholipid |
90 % |
|
|
ii. |
Carotenoids, Cholesterol, Vitamin E |
10 % |
|
|
03 |
RNA |
0.5 % |
|
|
04 |
DNA |
Few Amount |
|
|
05 |
Sulphur, Iron, Copper, Calcium, Magnesium and some other Vitamins |
Few Amount |
|
|
06 |
Enzymes |
Few Amount |
|
- According to Warburz (1913), enzymes related to respiration are also found in mitochondria, which was confirmed by Schneider, Palade and Hogeboom in 1948. These enzymes are as -
- In Outer Membrane - Monoamine Oxidase, NADH - Cytocrome c. reductase
- In Mitochondrial Space - Adenylatekinase , Nucleoside diphosphokinase
- In Inner Membrane - Succinate dehydrogenease
- In Matrix - Malate , Isocitrate dehydrogenase
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