Mitochondria : Discovery , Morphology & Chemical Composition

1. Discovery & Occurance 

  • First observed by Kölliker in 1880 in the striated muscles of insects.
  • Mitochondria were actually discovered by W. Fleming and R. Altman.
  • According to Fleming (in 1882), mitochondria are thread-like structures, which he named Phyla.
  • Altman observed them in cells in 1890 and referred to them as granular particles and named them Bioplasts. That is, a thread made of granules.
  • Mitochondria was named by C. Benda. Mitochondria is made up of two words, Mitos and Chondrion. Mitos means thread, and Chondria means granule. That is, a thread made of granules.
Mitochondria

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  • Ben-sley & Hoerr isolated mitochondria from liver cells in 1934.
  • Their presence in plant cells was discovered in 1904 by F. Meves in a plant called Nymphaea.
  • Michalis in 1898 discovered that mitochondria plays an important role in respiration and told that these are the centers of the Cellular Respiration.
  • All the mitochondria present in a single cell are collectively called a Chondriosome
    • These are also known as Chondrioplast , Plasmosome , Plastosome and Plastochondrian.
  • Except bacteria and blue-green algae , Mitochondria are present in every cells of plants and animals of living organisms.

2. Morphology Of The Mitochondria

  • Mitochondria seems like Rod-shaped , Filamentous , Spherical and Granular .
  • The morphology of mitochondria is different in every cell. 
  • The perimeter of the mitochondria is 0.5 - 2 µm. However, depending on their ability to function in certain conditions, they are found up to 7 µm in size.
  • The perimeter of the mitochondria is 0.5 - 2 µm.
  • The average length of Mitochondria is 3 - 5 µm and the diameter is 0.5 - 2 µm .
  • Depending on the cell's function and volume, the number of mitochondria in a cell can range from 50 to 50,000. Animal cells contain more mitochondria than plant cells.
    • Algae cells contains only one mitochondria.
    • Yeast cells contains less than 10 mitochondria.
    • Amoeba contains approximately 50,000 mitochondria.
    • Liver cells possess 1000 - 1600 mitochondria. Liver cells of Rat Possess 1000 to 2500 mitochondria.
    • Sea urchin eggs have 1,50,000 to 3,000,000 mitochondria.
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3. Chemical Composition

  • Mitochondria contain 65-70% proteins, of which 30% are structural proteins and 40% are functional proteins.
  • Mitochondria contain 25-30% lipids, of which 90% are phospholipids and the remaining 10% are Carotenoids, Cholesterol, Vitamin E, etc.
  • Sulphur, Iron, Copper, Calcium, Magnesium and some other Vitamins are also found in trace amounts.
  • 70 S Ribosomes are found in mitochondria.
  • DNA , RNA and RNA Polymerase etc. are also present in mitochondria.

Sr. No.

Chemical Substances

Quantity ( in % )

01

 

Protein

65 – 70 %

i.

Structural Protein

30 %

ii.

Enzymatic Protein

40 %

02

Lipid

25 – 30 %

i.

Phospholipid

90 %

ii.

Carotenoids, Cholesterol, Vitamin E

10 %

03

RNA

0.5 %

04

DNA

Few Amount

05

Sulphur, Iron, Copper, Calcium, Magnesium and some other Vitamins

Few Amount

06

Enzymes

Few Amount

  • According to Warburz (1913), enzymes related to respiration are also found in mitochondria, which was confirmed by Schneider, Palade and Hogeboom in 1948. These enzymes are as - 
    • In Outer Membrane - Monoamine Oxidase, NADH - Cytocrome c. reductase
    • In Mitochondrial Space - Adenylatekinase , Nucleoside diphosphokinase 
    • In Inner Membrane - Succinate dehydrogenease
    • In Matrix - Malate , Isocitrate dehydrogenase

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